ABSTRACT
Objective To explore the expression and biological significance of resistance gene of GST Pi,ToPoII and PgP in breast carcinoma. Method To test and analyse the resistance gene for GST Pi,ToPoII and PgP of 52 cases of breast carcinoma was made by using instant microwave heating immunohistochemistry.Results (1) Positive expression rates of GST Pi,ToPoII and PgP in 52 cases of breast carcinoma were 50.0% (26/52), 17.3% (9/52) and 11.5%(6/52),respectively. (2) Invasive ductal carcinoma had positive expression of three resistance genes at different levels,and the resistance genes in invasive ductal carcinoma were more widely than those in other types breast cancer. (3) There was no obvious relationship between positive expression of GST Pi,ToPoII and PgP in breast carcinoma with patients' age. However,the positive expression of three resistance genes in patients aged 40 to 49 and aged 60 to 69 were higher than other ages.Conclusions (1) The multidrug resistance genes existing in some patients of breast carcinoma or the primary cancer cell has resistance to GST Pi,ToPoII and PgP antineoplastics. (2) Before chemotherapy to patients with breast carcinoma, the test of resistance gene should be done, which will help doctors select a resonable method of combination of drugs so as to improve curative effect and to prolong patients' life.
ABSTRACT
Objective To be investigated the morphology of colorectal small flat adenoma and the expressions of p53 , p21, ER and PR. Methods Colonoscopy ( Olympus CF 240) and microscopy ( Olympus BV 41) were used to observe 50 cases of colorectal small flat adenomas. The expressions of p53 , p21 , ER, and PR were detected by the two steps of immunohistochemistry in 50 cases of small flat adenomas and the surrounding mucosa, 26 cases of colorectal carcinomas, while 15 cases of the normal colorectal mucosa as control group. Results These lesions were distributed throughout the large bowel, the prevalence in order was transverse colon, sigmoid and rectum. The small flat adenoma was round, flat, or sessile in shape, and sized